Transcriptome analysis at the single cell level from frozen tissue is
Single nucleus transcriptome analysis (single nucleus analysis) is a method to analyze the entire transcriptome from a single nucleus. This cutting-edge technology can analyze tissue which cannot be assessed by single cell transcriptome analysis (single cell analysis). Single nucleus analysis uses frozen tissue to identify pathophysiological mechanisms of diseases that are difficult to identify with conventional single cell analysis. In addition to single cell analysis, Axcelead DDP has established a novel technique for preparing brain tissue nuclei which has been difficult to implement until now. Furthermore, drug discovery research, such as the identification of novel targets and biomarkers based on the transcriptome analysis, has accelerated with the support of experienced bioinformaticians through their consideration of the analysis results.
Now it is possible to handle tissue
that could not be assessed by single cell analysis.
What is possible with single nucleus analysis？
1．A New Approach to Identify Pathophysiological Mechanisms
- Analysis at the genetic and cellular level is now possible by using postmortem frozen brain tissue from valuable clinical specimens.We support deeper understanding of physiological and pathological mechanisms in the central nervous system.
Rett syndrome: Nature Neuroscience 2018; 21(12):1670–1679
Alzheimer’s disease: Nature 2019; 570(7761):332-337
- Tissue which contains cells that are easily damaged, such as the liver, can be analyzed more accurately by using frozen tissue.
OMICS 2020; 24(4):180-194
- Even for diseases for which changes in the pathway for individual cell types were unknown due to sample limitations, knowledge of each cell type in each pathological stage can be obtained.
Patients with early diabetic nephropathy: PNAS 2019; 116(39):19619-19625
2．Realization of a flexible test plan
- Since frozen tissue can be used, limitations in conventional single cell analysis, such as “analysis must be performed on the same day as sampling,” no longer apply. As a result, transcriptome analysis can be performed more easily.
This also contributes to research efficiency and convenience.
Please feel free to contact Axcelead DDP for drug discovery research using single nucleus analysis!
Exploration of new drug targets and biomarkers through identification of pathological mechanisms
【Related services】Single cell transcriptome analysis