Biology

We offer the best solutions to biological evaluation at any drug discovery stage from target/biomarker identification through IND-enabling studies.
 
▶Integrated Translational Science
▶Technologies for biological evaluation
 
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Pharmacology studies in oncology
In vitro studies
Cancer cell proliferation assays	▶ Cell panel
	▶ 3D cultures (spheroid culture, soft agar culture)
	▶ Combination effect analysis
Cancer immunology-related analysis	▶ T cell cytotoxicity assay
	▶ Antibody-dependent cellular cytotoxicity (ADCC) assay
Target validation, Mechanism of action analysis, biomarker analysis
In vivo studies
Antitumor efficacy studies	▶ In vivo combination study
	▶ In vivo radiotherapy study
	▶ Pharmacological effect evaluation by tumor volume, survival curve, and in vivo imaging
Target engagement, pharmacokinetic/pharmacodynamic (PK/PD) analysis: blood, tumor, mouse organs
Tumor-infiltrating lymphocyte (TIL) analysis	▶ Flow cytometry, immunohistochemistry (IHC), and single-cell RNA-seq analysis
Animal models	▶ Immunodeficient mouse model (xenograft model)
	・　Human cancer cell lines / patient-derived tumor (PDx)
	・　Subcutaneous tumor, orthotopic tumor, metastasis, systemic dissemination models
	▶ Immunocompetent mouse model (syngeneic model)
	・　Mouse cancer cell lines
	・　Subcutaneous tumor, metastasis models
Pharmacology studies in immunological disorders
In vitro studies
Analyses of cytokine production, signal activation, and cell types in primary culture cells and whole blood
Proliferation assays of T cells, B cells, etc.
Th1, Th17, and Treg differentiation assays
Migration and apoptosis assays of splenocytes, thymocytes, B cells, neutrophiles, etc.
Target validation, Mechanism of action analysis, Biomarker analysis
In vivo studies
Target engagement and PK/PD analysis: Cytokine production and intracellular signal activation, etc.
Immune cell phenotyping in blood, lymph nodes, and various other tissues (Flow cytometry using 5-laser BD LSRFortessaTM)
Antibody production assay
Animal models	▶ Models of inflammatory bowel diseases
	▶ Multiple sclerosis model
	▶ Rheumatoid arthritis model
	▶ Psoriasis models, etc.
Pharmacology studies in central nervous system diseases
In vitro studies
Protein and gene expression analysis in primary central nervous system cells
In vivo studies
Behavioral test	▶ Psychiatric-disease-related
	・　Prepulse inhibition test
	・　3-chamber sociability test
	・　Psychostimulant-induced hyperactivity
	▶ Motor function
	・　Grip strength test
	・　Rotarod
	・　Running wheel test
	▶ Cognitive function
	・　Novel object recognition test
	・　8-Arm radial maze test
Target engagement and PK/PD analysis	▶ Microdialysis
	▶ Sleep EEG
	▶ Pathological analysis
	▶ Collection of cerebrospinal fluid (CSF)
Animal models	▶ Cognitive dysfunction models (scopolamine, MK-801)
	▶ Valproic acid-induced autism model
	▶ Poly (I:C) model of schizophrenia
	▶ Development of animal models by intracerebral injection
Pharmacology studies in cardiovascular diseases
In vitro studies
Hypertrophy, apoptosis, and cell signaling assay in primary cardiomyocyte
Isolated perfused rat heart (Langendorff perfused heart)
Aortic ring preparation
Target validation, Mechanism of action analysis, Biomarker analysis
In vivo studies
Target engagement and PK/PD analysis
CV safety (blood pressure, cardiac function, and electro-cardiogram, etc.)
Animal models	▶ Ischemia/reperfusion model
	▶ Hypertension models
	▶ Pulmonary hypertension model
	▶ Renal disease models
	▶ Heart failure models (TAC model, MI model, etc.)
	・　Cardiac function measurement by echocardiography and cardiac catheterization
Pharmacology studies in gastrointestinal motility disorders
In vitro studies
Magnus test
In vivo studies
Defecation studies
Bead expulsion assay
Colonic manometry test
Animal model	▶ Spinal cord injury model
Pharmacology studies in metabolic diseases
In vitro studies
Fibrosis evaluation in co-culture of hepatic stellate cell and hepatocyte
Glucose metabolism / lipid metabolism assay in primary hepatocyte, cell lines of hepatocyte, pancreatic β cell, and adipocyte
Lipid metabolism assay in isolated skeletal muscle
Target validation, Mechanism of action analysis, Biomarker analysis
MOA analysis of lowering/increasing blood glucose, body weight loss/gain
In vivo studies
Target engagement and PK/PD analysis
Animal models	▶ Non-alcoholic steatohepatitis (NASH) models
	▶ Diabetes models
	・　Glucose tolerance test, insulin tolerance test
	・　Glucose clamp technique
	▶ Obesity models
	・　Body composition analysis (Echo-MRI and X-ray CT)
	・　Energy expenditure analysis (Open-circuit indirect calorimeter)
Pharmacology studies for cell therapy using large animals
In vivo studies
Immunosuppressed models	▶ Immunosuppressed porcine/monkey model
Disease models	▶ Heart failure porcine/monkey model
	▶ Type 1 diabetes porcine model
Cell engraftment evaluation
Omics and Bioinformatics
Metabolomics	▶ Pathway analysis
	▶ Analytical method development for target metabolites
Lipidomics	▶ Non-target analysis covering over 20 lipid classes
Proteomics	▶ Protein turnover analysis
	▶ Protein-protein interaction analysis
	▶ Phospho-proteomics
	▶ Post-translational modification profiling
Data analysis	▶ MoA analysis using global omics data
	▶ Annotations (gene functions, mutation, etc.)
	▶ Data visualization
	▶ GWAS
	▶ Gene mutation analysis
	▶ Single-cell RNA-seq data analysis
	▶ Biomarker discovery
Consultation on omics data acquisition and validation study plan
Transcriptomics	▶ Ampli-seq
Genomics	▶ Target re-sequencing
	▶ 16S rRNA sequencing for microbiome analysis
Genetically modified animals (mice)
Generation of knock-out (KO) rodents
Generation of transgenic (Tg) rodents
Generation of knock-in (KI) rodents
Creation of humanized rodents
Additional gene modification to rodent disease models
Cleaning to SPF grade and breeding(Rodents introduction from outside organizations)
Cryopreservation of embryos and sperm of rodents
gRNA design and vector construction for genome

▶Pharmacology studies using immunosuppressed pigs