Biology
We offer the best solutions to biological evaluation at any drug discovery stage from target/biomarker identification through IND-enabling studies.
▶Integrated Translational Science
▶Technologies for biological evaluation
◇ Service menu for Biology
Pharmacology studies in oncology | |
In vitro studies | |
Cancer cell proliferation assays | ▶ Cell panel |
▶ 3D cultures (spheroid culture, soft agar culture) | |
▶ Combination effect analysis | |
Cancer immunology-related analysis | ▶ T cell cytotoxicity assay |
▶ Antibody-dependent cellular cytotoxicity (ADCC) assay | |
Target validation, Mechanism of action analysis, biomarker analysis | |
In vivo studies | |
Antitumor efficacy studies | ▶ In vivo combination study |
▶ In vivo radiotherapy study | |
▶ Pharmacological effect evaluation by tumor volume, survival curve, and in vivo imaging | |
Target engagement, pharmacokinetic/pharmacodynamic (PK/PD) analysis: blood, tumor, mouse organs | |
Tumor-infiltrating lymphocyte (TIL) analysis | ▶ Flow cytometry, immunohistochemistry (IHC), and single-cell RNA-seq analysis |
Animal models | ▶ Immunodeficient mouse model (xenograft model) |
・ Human cancer cell lines / patient-derived tumor (PDx) | |
・ Subcutaneous tumor, orthotopic tumor, metastasis, systemic dissemination models | |
▶ Immunocompetent mouse model (syngeneic model) | |
・ Mouse cancer cell lines | |
・ Subcutaneous tumor, metastasis models | |
Pharmacology studies in immunological disorders | |
In vitro studies | |
Analyses of cytokine production, signal activation, and cell types in primary culture cells and whole blood | |
Proliferation assays of T cells, B cells, etc. | |
Th1, Th17, and Treg differentiation assays | |
Migration and apoptosis assays of splenocytes, thymocytes, B cells, neutrophiles, etc. | |
Target validation, Mechanism of action analysis, Biomarker analysis | |
In vivo studies | |
Target engagement and PK/PD analysis: Cytokine production and intracellular signal activation, etc. | |
Immune cell phenotyping in blood, lymph nodes, and various other tissues (Flow cytometry using 5-laser BD LSRFortessaTM) | |
Antibody production assay | |
Animal models | ▶ Models of inflammatory bowel diseases |
▶ Multiple sclerosis model | |
▶ Rheumatoid arthritis model | |
▶ Psoriasis models, etc. | |
Pharmacology studies in central nervous system diseases | |
In vitro studies | |
Protein and gene expression analysis in primary central nervous system cells | |
In vivo studies | |
Behavioral test | ▶ Psychiatric-disease-related |
・ Prepulse inhibition test | |
・ 3-chamber sociability test | |
・ Psychostimulant-induced hyperactivity | |
▶ Motor function | |
・ Grip strength test | |
・ Rotarod | |
・ Running wheel test | |
▶ Cognitive function | |
・ Novel object recognition test | |
・ 8-Arm radial maze test | |
Target engagement and PK/PD analysis | ▶ Microdialysis |
▶ Sleep EEG | |
▶ Pathological analysis | |
▶ Collection of cerebrospinal fluid (CSF) | |
Animal models | ▶ Cognitive dysfunction models (scopolamine, MK-801) |
▶ Valproic acid-induced autism model | |
▶ Poly (I:C) model of schizophrenia | |
▶ Development of animal models by intracerebral injection | |
Pharmacology studies in cardiovascular diseases | |
In vitro studies | |
Hypertrophy, apoptosis, and cell signaling assay in primary cardiomyocyte | |
Isolated perfused rat heart (Langendorff perfused heart) | |
Aortic ring preparation | |
Target validation, Mechanism of action analysis, Biomarker analysis | |
In vivo studies | |
Target engagement and PK/PD analysis | |
CV safety (blood pressure, cardiac function, and electro-cardiogram, etc.) | |
Animal models | ▶ Ischemia/reperfusion model |
▶ Hypertension models | |
▶ Pulmonary hypertension model | |
▶ Renal disease models | |
▶ Heart failure models (TAC model, MI model, etc.) | |
・ Cardiac function measurement by echocardiography and cardiac catheterization | |
Pharmacology studies in gastrointestinal motility disorders | |
In vitro studies | |
Magnus test | |
In vivo studies | |
Defecation studies | |
Bead expulsion assay | |
Colonic manometry test | |
Animal model | ▶ Spinal cord injury model |
Pharmacology studies in metabolic diseases | |
In vitro studies | |
Fibrosis evaluation in co-culture of hepatic stellate cell and hepatocyte | |
Glucose metabolism / lipid metabolism assay in primary hepatocyte, cell lines of hepatocyte, pancreatic β cell, and adipocyte | |
Lipid metabolism assay in isolated skeletal muscle | |
Target validation, Mechanism of action analysis, Biomarker analysis | |
MOA analysis of lowering/increasing blood glucose, body weight loss/gain | |
In vivo studies | |
Target engagement and PK/PD analysis | |
Animal models | ▶ Non-alcoholic steatohepatitis (NASH) models |
▶ Diabetes models | |
・ Glucose tolerance test, insulin tolerance test | |
・ Glucose clamp technique | |
▶ Obesity models | |
・ Body composition analysis (Echo-MRI and X-ray CT) | |
・ Energy expenditure analysis (Open-circuit indirect calorimeter) | |
Pharmacology studies for cell therapy using large animals | |
In vivo studies | |
Immunosuppressed models | ▶ Immunosuppressed porcine/monkey model |
Disease models | ▶ Heart failure porcine/monkey model |
▶ Type 1 diabetes porcine model | |
Cell engraftment evaluation | |
Omics and Bioinformatics | |
Metabolomics | ▶ Pathway analysis |
▶ Analytical method development for target metabolites | |
Lipidomics | ▶ Non-target analysis covering over 20 lipid classes |
Proteomics | ▶ Protein turnover analysis |
▶ Protein-protein interaction analysis | |
▶ Phospho-proteomics | |
▶ Post-translational modification profiling | |
Data analysis | ▶ MoA analysis using global omics data |
▶ Annotations (gene functions, mutation, etc.) | |
▶ Data visualization | |
▶ GWAS | |
▶ Gene mutation analysis | |
▶ Single-cell RNA-seq data analysis | |
▶ Biomarker discovery | |
Consultation on omics data acquisition and validation study plan | |
Transcriptomics | ▶ Ampli-seq |
Genomics | ▶ Target re-sequencing |
▶ 16S rRNA sequencing for microbiome analysis | |
Genetically modified animals (mice) | |
Generation of knock-out (KO) rodents | |
Generation of transgenic (Tg) rodents | |
Generation of knock-in (KI) rodents | |
Creation of humanized rodents | |
Additional gene modification to rodent disease models | |
Cleaning to SPF grade and breeding(Rodents introduction from outside organizations) | |
Cryopreservation of embryos and sperm of rodents | |
gRNA design and vector construction for genome |
▶Pharmacology studies using immunosuppressed pigs