Pharmacology studies using immunosuppressed pigs

Pharmacology studies using immunosuppressed pigs are now available!

In the field of regenerative medicine, we can now offer you pharmacology studies using immunosuppressed pigs with data that can be highly clinically extrapolated.
We contribute to your success in clinical studies.

In the field of regenerative medicine, it is desirable to verify the efficacy and safety of regenerated organs produced with human cells, using large animals in which it is possible to use the same interventions as in clinical use, before they are transplanted into humans. On the other hand, when a regenerated organ produced with human cells is transplanted into a pig, which is similar in body size to humans, an immune response to xenograft occurs. Before this immunosuppressed pig model was developed, it was considered impossible to verify the safety and efficacy of human cell-derived regenerative medicine products with pigs.
We, Axcelead Drug Discovery Partners (hereinafter referred to as Axcelead DDP), adopt surgical procedures and pharmacokinetic(PK) measurement of immunosuppressants as a basic procedure to prepare immunosuppressed pigs with data that can be highly clinically extrapolated, by controlling the level of immunosuppression appropriate for individual animals, supporting your success in clinical studies.

Preparation of the immunosuppressed pig model

Examples of pathological model preparation

◆ Immunosuppressed heart failure pig model:

◆ Immunosuppressed type 1 diabetes mellitus pig model:

Please consult with us about the preparation of other pathological models.

■ A study on the production of the immunosuppressed pig model co-authored by Toshiyuki Maki and Keiko Igaki, researchers at Axcelead DDP, has been published in “Nature Research Protocol Exchange”.
“Surgically produced, controllable immunocompromised pigs”
(information on the publication by Nature Research Protocol Exchange)
Eiji Kobayashi et al., Posted 03 Oct 2019

Applications in the field of regenerative medicine

At the International Society for Stem Cell Research (ISSCR) Annual Meeting, the results of a study in which human iPS cell-derived cardiomyocytes were successfully grafted in the heart of an immunosuppressed pig model were presented on a poster.

Norihisa Tamura (T-CiRA Discovery, Takeda Pharmaceutical Company Limited), et al.
The study was co-authored by Nobuyuki Amano, Manami Kaneko, Toshiyuki Maki, Noriyasu Sano, and Teruki Hamada from Axcelead DDP.

<Summary of the presentation>

For patients with severe heart failure, heart transplantation is their final means of survival, awaiting the development of new treatment options. Human iPS cell-derived cardiomyocyte transplantation is expected to be one of the treatment options. The size and heart rate of small animal hearts are significantly different to those of humans. The clinical efficacy should be evaluated in large animals. Since it is essential to produce an immunosuppressed large animal model for the grafting of human-derived cells, we investigated optimal types and dosages of immunosuppressants. In a comparison between cyclosporin and tacrolimus, clinically-used calcineurin inhibitors, cyclosporin was more effective in pigs based on the results of the studies on porcine PBMC proliferation and measurement of in vivo PK profiles. A pig underwent surgical removal of its immune system organs, was administered several immunosuppressants including cyclosporin, and then human iPS cell-derived cardiomyocytes were transplanted into the heart. The successful engraftment of human-derived cardiomyocytes in the heart was confirmed about 3 weeks later.

<Remarkable advantages of immunosuppressed pig model used in the study!>

Please contact Axcelead DDP for evaluation systems on regenerative medicine research!

MenuStudy contents
cell RNA-seq analysisevaluation of gene expression changes over time
Metabolomics/ proteomics of cells and mediafactors and prediction markers for cell differentiation
Low molecular weight compound screeningPhenotypic screening using iPS cells
Efficacy study/ engraftment evaluation study/ safety study using pathological model animal modelss・Large animals ( pig、monkey)
・Small animals (mouse, rat)
OtherEvaluation of Cell Delivery Device

Please contact us for evaluation systems not in the table shown above.

Axcelead offers pharmacology studies using immunosuppressed pig models
  • Biology